Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors.
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A genome-wide scanning of Sorghum bicolor resulted in the identification of 25 SbHsfgenes. Phylogenetic analysis shows the ortholog genes that are clustered with only rice, representing acommon ancestor. Promoter analysis revealed the identification of different cis-acting elements thatare responsible for abiotic as well as biotic stresses. Hsf domains like DBD, NLS, NES, and AHAhave been analyzed for their sequence similarity and functional characterization. Tissue specific expressionpatterns of Hsfs in different tissues like mature embryo, seedling, root, and panicle were studiedusing real-time PCR. While Hsfs4 and 22 are highly expressed in panicle, 4 and 9 are expressed inseedlings. Sorghum plants were exposed to different abiotic stress treatments but no expression of any Hsf was observedwhen seedlings were treated with ABA. High level expression of Hsf1 was noticed during high temperature as well ascold stresses, 4 and 6 during salt and 5, 6, 10, 13, 19, 23 and 25 during drought stress. This comprehensive analysis ofSbHsf genes will provide an insight on how these genes are regulated in different tissues and also under different abioticstresses and help to determine the functions of Hsfs during drought and temperature stress tolerance
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