Structural characterization of the DRF1 gene of Aegilops speltoides and comparison of its sequence with those of B and other Triticeae genomes uri icon

abstract

  • The genusAegilopsL. has been intensively investigated due to its close relationship with wheat (TriticumL.) as contributor of B and D subgenomes. Because of their vast genetic diversity,Aegilopsspecies represent a rich source of alleles of agronomic interest, which could be used to widen the wheat gene pool and improve tolerance to diseases, pests, drought, cold and other environmental stresses. We report the isolation and characterization of theDehydration Responsive Factor 1(DRF1)gene in three accessions ofAe. speltoidescoming from different regions of the Fertile Crescent. TheDRF1gene belongs to theDREBgene family and encodes transcription factors which play a key role in plant response to water stress. As in other cereals, theDRF1gene inAegilops speltoidesconsists of four exons and three introns and undergoes alternative splicing. A processed pseudogene was also identified and compared with the sequence of an actual mRNA transcript, breaking new ground in the understanding of the complex regulation mechanism of this gene. The genetic diversity was evaluated by comparison of inter- and intra-species variation among someAegilopsandTriticeae, by considering both the whole gene and exon 4 sequences. The phylogenetic analyses were able to cluster the sequences in well-supported clades attributable to the genomes analysed. The overall results suggest that there is a high similarity between the B and S genome copies of theDRF1gene but also features indicating that the two genomes have evolved independently.

publication date

  • 2020
  • 2020