Screening and characterisation of virus causing yellow leaf disease of Tephrosia in Ethiopia uri icon

abstract

  • Tephrosia, an important medicinal plant, and a potential livestock feed, was found to be affected by a leaf yellowing disease at the Ziway field site of the International Livestock Research Institute. A total of fifty samples from 300 plants were collected from twentyTephrosia speciesin three consecutive planting seasons; 2015, 2016 and 2017. The samples were screened for viral infection by dot-blot assay with antiserum targetting eight viruses. RT-PCR of dot-blot positive samples using virus specific primers gave an amplification product of the expected size (867 bp) only forcucumber mosaic virus(CMV) inTephrosia senna. The amplified products were sequenced; coat protein sequence (657 bp) extracted, and submitted to NCBI database (Tep-Et; KY041651). Sequence alignment and phylogenetic analyses indicated that the isolate, Tep-Et, shared maximum identity [88.8-97.5% nucleotide (nt) and 89.4-96.3% amino acid (aa)] with CMV belonging to members of subgroup-I. To our knowledge, this is the first report of molecular characterisation of a CMV isolate infecting a new host,T. sennain Ethiopia.

publication date

  • 2020
  • 2020