Insights into the genome sequence of ovine Pasteurella multocida type A strain associated with pneumonic pasteurellosis uri icon

abstract

  • P. multocida type A strain NIVEDI/PMS-1 genome was 2,543,931bp in size, assembled into 67 contigs, consisted of 1879 protein-coding regions, 19 genomic islands (GIs) and 10 phage regions. The identified GIs and phage regions showed high sequence identity mostly with other Pasteureilaceae family respiratory pathogens indicating horizontal gene transfer in ovine respiratory niche. The phylogenetic analysis revealed strain NIVEDI/PMS-1 as closest neighbor to P. multocida P1933 strain reported from bovine. Comparative genomic analysis of strain NIVEDI/PMS-1 with virulent GX-Pm and avirulent Pm70 strains revealed presence of 45 virulence genes common with virulent strain. Further, various virulence factors and accessory genes needed for survival and pathogenesis were also identified. Dermonecrotic toxin (ToxA) like protein presence in the strain appears interesting and further study is needed to understand its role in ovine pasteurellosis.
  • Pasteurella multocida is a causative agent of pneumonic pasteurellosis in ruminants. Bovine and porcine strains of P. multocida have been sequenced and analyzed worldwide. However limited genomic information is available on ovine strains involved in causation of pneumonic pasteurellosis. Therefore, the present study focused on genome sequencing and analysis of a P. multocida type A isolate recovered from respiratory infection of sheep to unravel the genetic contents and its involvement in the disease.

publication date

  • 2018
  • 2018