β-Cryptoxanthin–Biofortified Hen Eggs Enhance Vitamin A Status When Fed to Male Mongolian Gerbils uri icon

abstract

  • Background: Consumption of provitamin A carotenoid biofortified crops, such as maize, supports vitamin A (VA) status in animals and humans. Laying hens that consume ss-cryptoxanthin-biofortified maize deposit ss-cryptoxanthin into egg yolk.
  • Conclusions: The preformed retinol in the eggs enhanced gerbil VA status, and the ss-cryptoxanthin-biofortified eggs from hens fed orange maize prevented deficiency. Biofortified maize can enhance VA status when consumed directly or through products from livestock fed orange maize.
  • Methods: ss-Cryptoxanthin-biofortified egg yolk, produced in hens fed biofortified orange maize or tangerine-fortified maize feeds, was freeze-dried and fed to gerbils. White-yolked eggs were produced by feeding white maize to hens. Gerbils (n = 57) were fed VA-deficient feed for 28 d. After baseline (n = 7), treatments (n = 10/group) included oil control (VA-); 16.7% orange maize-biofortified, tangerine-fortified, or white-yolk egg feeds; or retinyl acetate as positive control (VA+) matched to daily preformed retinol intake from the eggs for 30 d. Preformed retinol did not differ between the egg yolks. Gerbil liver retinol, lipid, fatty acids, and cholesterol were determined.
  • Objective: We investigated whether ss-cryptoxanthin-biofortified egg consumption would affect VA status of male Mongolian gerbils (Meriones unguiculatus) compared with white-yolked eggs.
  • Results: Liver retinol concentration (0.13 +/- 0.03 mu mol/g) and total hepatic VA (0.52 +/- 0.12 mu mol) were higher in gerbils fed orange maize-biofortified eggs than in all other groups. The VA-group was severely VA deficient (0.018 +/- 0.010 mu mol/g; P < 0.05). Liver retinol was similar among VA+, tangerine-egg-, and white-egg-fed gerbils, but retinol reserves were higher in tangerine-egg-fed gerbils (0.35 +/- 0.11 mu mol) than in VA+ or VA-gerbils or at baseline (P < 0.05). Liver fat was 3.6 times (P < 0.0001) and cholesterol was 2.1 times (P < 0.004) higher in egg-fed groups that experienced hepatosteatosis. Liver fatty acid profiles reflected feed, but retinyl ester fatty acids did not.

publication date

  • 2018
  • 2018