Responses of cassava (Manihot esculenta Crantz) varieties to in vitro mannitol-induced drought stress uri icon

abstract

  • Rapid selection of drought-tolerant cassava varieties by in vitro screening is essential for efficient breeding for drought tolerance. The objectives of this study were to describe the morphological response of 12 cassava cultivars to in vitro mannitol-induced drought stress and identify tolerant ones. Plantlets were raised from nodal segments on Murashige and Skoog (MS) medium containing either 0 g/L or 20 g/L mannitol. Mannitol increased the number of shoots of plantlets per explant (NSS) of all cultivars by an average of 18.2%. However, mannitol decreased the dry shoot weight (DSW) of all cultivars by half. Mannitol decreased total number of leaves (TNL) of plantlets by more than half in all cultivars, except three, in which mannitol had no effect on the TNL. The average root length (ARL) of each one of the following four varieties: TMS 30572, TMS 50395, MM 96/1751, and TMS 94/0026, was not sensitive to mannitol treatment. However, mannitol increased the ARL of MM 98/3437, TMS 94/0039, TMS 92B/00061, TMS 91934 and TMS 84/00353 but decreased the ARL of TMS 05/1654, TME 419, and TMS 92/0326. Mannitol had no effect on dry root weight (DRW) of plantlets of TMS 91934, TMS 94/0026, TME 419, TMS 30572, TMS 50395, and MM 98/3437. The DRW of each of the following four varieties: TMS 05/1654, TMS 92/0326, TMS 92B/00061, and TMS 94/0039, was decreased by mannitol, whereas the DRW of each of TMS 84/00353 and MM 96/1751 was increased by an average of 42.4%. Stress-tolerance index showed that TMS 84/00353, MM96/1751, MM98/3437, and TMS 94/0026 were tolerant of mannitol-induced drought stress and, therefore, recommended for planting in drought-prone regions by farmers.

publication date

  • 2018
  • 2018