Physiological parameters of seed vigour in ex situ stored sorghum germplasm uri icon

abstract

  • Viability and vigour are seed quality parameters that affect the seed chain. Seed vigour is a measure ofaccumulated damage in seed as viability declines from physiological maturity. This study aimed atdetermining the seed vigour of various sorghum genotypes stored ex situ for 10 years using the HeatShock Stress Test (HSST), Cold Test (CT), Standard Germination Test (SGT) and field germination. Thesamples from the regeneration trial were kept in ex situ storage at 0-4°C and 40% relative humidity from2003 to 2014. The experiments were arranged in a completely randomised design with four replicationand differences in radicle length, shoot length for the 65 genotypes evaluated at 5% level ofsignificance. An unpaired T- test was used to compare the accuracy of the quality methods inpredicting field emergence. All the evaluated genotypes responded differentially to all the vigour testmethods. There were significant differences (P<0.01) in the performance of genotypes with respect toradical and shoot length in response to the CT, HSST and the SGT. The results of CT and HSSTpredicted field emergence better than the standard germination test. None of the quality test methodsand a strong and significant correlation with field emergence. Genotypes IS 30260 and IS 34637 werevigourous as they had the highest shoot length of 43.75 and 42.38 mm and IS 30063 had 45.52 mm inroot length after 48 h of incubation. Overall, this study showed that field emergence of sorghum cannotbe accurately predicted from a standard germination, HSST and CT

publication date

  • 2015
  • 2015