Insecticidal activity of a novel fatty acid amide derivative from Streptomyces species against Helicoverpa armigera uri icon

abstract

  • Helicoverpa armigera, an important pest causes serious damageto grain legumes. The main objective of this study was to isolateand identify the metabolite against H. armigera from a previouslycharacterised Streptomyces sp. CAI-155. The culture filtrate of CAI-155 was extracted using Diaion HP-20 and the active fractions werefractionated on Silica and C18 column chromatography. The C18active fraction was further fractionated on Silica gel 60 F254 thin layerchromatography (TLC). The most active fraction (Rf 0.64) purified fromTLC led to the identification of a novel metabolite N-(1-(2,2-dimethyl-5-undecyl-1,3-dioxolan-4-yl)-2-hydroxyethyl)stearamide by spectralstudies. The purified metabolite showed 70?78% mortality in 2ndinstar H. armigera by diet impregnation assay, detached leaf assay andgreenhouse assay. The LD50 and LD90 values of the purified metabolitewere 627 and 2276 ppm, respectively. Hence, this novel metabolitecan be exploited for pest management in future

publication date

  • 2016
  • 2016