Production and characterization of monoclonal antibodies for aflatoxin B1. uri icon

abstract

  • Hybridomas that secreted antibodies for aflatoxin B1 were selected usingtwo immunization protocols referred to as A and B. Protocol A is a standardimmunization method and resulted in the selection of only two clones that producedmonoclonal antibodies against aflatoxin B1. In protocol B a unique immunizationschedule which resulted in the generation of 10 hybridomas is described. Of the10, one antibody was highly specific to B1, four antibodies reacted equally stronglywith B1, G1 and weakly with B2. Another four reacted strongly with B1 andweakly with B2 and G1. One clone reacted equally strongly with B1, G1 and B2.Interestingly all the 10 antibodies showed little or no cross-reaction with G2

publication date

  • 1999
  • 1999