The relationship between graft incompatibility and phenols in Uapaca kirkiana Müell Arg. uri icon

abstract

  • The objective of the study was to determine the relationship between phenols and graft incompatibility in Uapaca kirkiana. Phenol quantification and identification were carried out using Folin-Ciocalteau reagent procedure, fluorescence microscopy and reverse phase high performance liquid chromatograph (RP-HPLC) above, below and at the graft union. Results showed no vascular cambium continuity above the scion/stock unions. Significant differences in total soluble phenols and cell wall bound phenols were obtained. Fluorescence microscope indicated the presence of flavonoids and other polymers above the union. The RP-HPLC identified ferulic acid as a major phenol component found in U. kirkiana plant cells and responsible for wood discolouration. High phenol concentrations were obtained in less compatible combinations than in compatible combinations. High peaks of -coumaric acid were obtained above the union. It is concluded that phenols, especially -coumaric acids and flavonoids caused poor callus formation at the union, and hence implicated in graft incompatibility
  • The objective of the study was to determine the relationship between phenols and graft incompatibility in Uapaca kirkiana. Phenol quantification and identification were carried out using Folin-Ciocalteau reagent procedure, fluorescence microscopy and reverse phase high performance liquid chromatograph (RP-HPLC) above, below and at the graft union. Results showed no vascular cambium continuity above the scion/stock unions. Significant differences in total soluble phenols and cell wall bound phenols were obtained. Fluorescence microscope indicated the presence of flavonoids and other polymers above the union. The RP-HPLC identified ferulic acid as a major phenol component found in U. kirkiana plant cells and responsible for wood discolouration. High phenol concentrations were obtained in less compatible combinations than in compatible combinations. High peaks of p-coumaric acid were obtained above the union. It is concluded that phenols, especially p-coumaric acids and flavonoids caused poor callus formation at the union, and hence implicated in graft incompatibility. (C) 2008 Elsevier B.V. All rights reserved.

publication date

  • 2008
  • 2008
  • 2008