Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum) uri icon

abstract

  • A linkage map, primarily based on SSCP-SNP markers, was constructed using 188 F2:3 mapping population progenies produced from a cross between two pearl millet inbred lines having diverse parentage. The skeleton linkage map covered 1019?cM and it comprised of 44 markers distributed across the seven linkage groups. Average adjacent-marker intervals ranged from 14?cM on LG1 to 38?cM on LG6, with an overall mean of 23?cM. Using the F2 linkage map and phenotypic data from the F2 and F2:3 generations of the mapping population, a total of 18 putative QTLs were detected for the three sink-size components. Eight QTLs explained 42.7% of observed phenotypic variation for panicle length using the F2:3 data set. For panicle diameter, 5 QTLs explained 45.8% of observed phenotypic variation. Similarly for grain size, 5 QTLs explained 29.6% of phenotypic variation. Genomic regions associated with panicle length, panicle diameter, and grain size were comapped on LG6 between Xpsms88 and Xpsms2270, indicating the existence of a gene or gene cluster. The QTLs for panicle length on LG2 and LG6 ( in both F2 and F2:3 data sets), for panicle diameter on LG2 and LG3 ( in the F2:3 data set), and for grain size on LG3 and LG6 ( in both F2 and F2:3 data sets) were identified as promising candidates for validation prior to possible application in marker-assisted breeding

publication date

  • 2013
  • 2013
  • 2013