Marker-assisted backcrossing to introgress resistance to fusarium wilt race 1 and ascochyta blight in C 214, an elite cultivar of chickpea uri icon

abstract

  • Fusarium wilt (FW) and Ascochyta blight (AB) are two major constraints to chickpea (Cicer arietinum L.) production. Therefore, two parallel marker-assisted backcrossing (MABC) programs by targeting foc1 locus and two quantitative trait loci (QTL) regions, ABQTL-I and ABQTL-II, were undertaken to introgress resistance to FW and AB, respectively, in C 214, an elite cultivar of chickpea. In the case of FW, foreground selection (FGS) was conducted with six markers (TR19, TA194, TAA60, GA16, TA110, and TS82) linked to foc1 in the cross C 214 x WR 315 (FW-resistant). On the other hand, eight markers (TA194, TR58, TS82, GA16, SCY17, TA130, TA2, and GAA47) linked with ABQTL-I and ABQTL-II were used in the case of AB by deploying C 214 x ILC 3279 (AB-resistant) cross. Background selection (BGS) in both crosses was employed with evenly distributed 40 (C 214 x WR 315) to 43 (C 214 x ILC 3279) SSR markers in the chickpea genome to select plant(s) with higher recurrent parent genome (RPG) recovery. By using three backcrosses and three rounds of selfing, 22 BC3F4 lines were generated for C 214 x WR 315 cross and 14 MABC lines for C 214 x ILC 3279 cross. Phenotyping of these lines has identified three resistant lines (with 92.7-95.2% RPG) to race 1 of FW, and seven resistant lines (with 81.7-85.40% RPG) to AB that may be tested for yield and other agronomic traits under multilocation trials for possible release and cultivation.
  • Fusarium wilt (FW) and Ascochyta blight (AB) are two majorconstraints to chickpea (Cicer arietinum L.) production. Therefore,two parallel marker-assisted backcrossing (MABC) programsby targeting foc1 locus and two quantitative trait loci (QTL)regions, ABQTL-I and ABQTL-II, were undertaken to introgressresistance to FW and AB, respectively, in C 214, an elite cultivarof chickpea. In the case of FW, foreground selection (FGS) wasconducted with six markers (TR19, TA194, TAA60, GA16, TA110,and TS82) linked to foc1 in the cross C 214 × WR 315 (FWresistant).On the other hand, eight markers (TA194, TR58, TS82,GA16, SCY17, TA130, TA2, and GAA47) linked with ABQTL-Iand ABQTL-II were used in the case of AB by deploying C 214× ILC 3279 (AB-resistant) cross. Background selection (BGS)in both crosses was employed with evenly distributed 40 (C214 × WR 315) to 43 (C 214 × ILC 3279) SSR markers in thechickpea genome to select plant(s) with higher recurrent parentgenome (RPG) recovery. By using three backcrosses and threerounds of selfing, 22 BC3F4 lines were generated for C 214 ×WR 315 cross and 14 MABC lines for C 214 × ILC 3279 cross.Phenotyping of these lines has identified three resistant lines (with92.7?95.2% RPG) to race 1 of FW, and seven resistant lines(with 81.7?85.40% RPG) to AB that may be tested for yield andother agronomic traits under multilocation trials for possible releaseand cultivation

publication date

  • 2014
  • 2014
  • 2014