Functional Genetic Diversity Analysis and Identification of Associated Simple Sequence Repeats and Amplified Fragment Length Polymorphism Markers to Drought Tolerance in Lentil (Lens culinaris ssp. culinaris Medicus) Landraces uri icon

abstract

  • Genetic diversity of 70 Mediterranean lentil (Lens culinaris ssp. culinaris Medicus) landraces was assessed using simple sequence repeats (SSRs) and amplified fragment length polymorphisms (AFLPs). These landraces were also assessed for variation in root and shoot traits and drought tolerance as estimated by relative water content (RWC), water losing rate (WLR) and wilting score (WS). Genetic diversity and clear differentiation of Moroccan landraces from those from northern Mediterranean regions (Italy, Turkey and Greece) were found. High genetic variation in root and shoot traits and traits related to drought tolerance was also observed. No relationship was found between drought tolerance of landraces and their geographic origin. Landraces with higher dry root biomass, chlorophyll content and root-shoot ratio were drought tolerant as evidenced by higher RWC and lower WLR and wilting severity. Kruskal-Wallis non-parametric test (K-W) was used to find SSRs and AFLPs associated with RWC, WLR and WS. Regression analysis showed six SSR and AFLP alleles explaining the highest phenotypic variation of RWC, WLR and WS (ranging from 21 to 50 % for SSRs and from 14 to 33 % for AFLPs). Functional genetic diversity analysis showed relationships between drought response of landraces and linked SSR and AFLP alleles to RWC, WLR and WS according to K-W test using canonical discriminant analysis. Our results confirm the feasibility of using association mapping to find DNA markers associated with drought tolerance in larger numbers of lentil landraces.
  • Genetic diversity of 70 Mediterranean lentil landraces was assessed using simple sequence repeat (SSR) and amplified fragment length polymorphisms (AFLP). Their variation for root and shoot traits and drought tolerance was evaluated using relative water content, water losing rate and wilting score. High level of genetic variation and clear differentiation of landraces from Morocco from those from northern Mediterranean originating from Italy, Turkey and Greece were found according to both SSR and AFLP techniques. High genetic variation for root and shoot traits as well as for drought tolerance was obtained. No correlation of drought response with landraces origin. Landraces with higher dry root biomass, chlorophyll content and root-shoot ratio were drought tolerant with higher relative water content and lower water losing rate and wilting severity. Kruskal-Wallis non- parametric test (K-W) was used to find associated SSRs and AFLPs to RWC, WLR and WS. Regression analysis showed six SSRs and AFLPs alleles explaining the highest phenotypic variation of RWC, WLR and WS. Functional genetic diversity based on drought response of landraces as estimated by RWC, WLR and WS was shown using SSRs and AFLPs alleles linked to these parameters according to K-W using canonical discriminant analysis. This highlighted the feasibility of association mapping studies aiming to find associated DNA markers with drought tolerance in larger number of lentil landraces

publication date

  • 2016
  • 2016
  • 2016