Callus Formation and Plant Regeneration from Protoplasts Derived from Suspension Culture of Rice (Oryza sativa L. cv. ‘Taipei 177’) uri icon

abstract

  • Protoplasts were isolated from young inflorescence-derived suspension cultures of a japonica rice cultivar "Taipei 177". The isolated protoplasts which were cultured either in liquid, agar on Sea plaque agarose underwent sustained division. Maximum plating efficiency of 1.06% occurred in a medium containing macroelements of KM, microelements and vitamins of B5, 0.5% Sea plaque agarose, 1.0 mg/l of 2,4-D, and glucose as an osmotic stabilizer. Green and albino plants were regenerated from the protocalli in MS semisolid medium containing 4 mg/l BAP, 0.5 mg/l NAA and 500 mg/l casein hydrolysate (MS18-2).

publication date

  • 1990
  • 1990
  • 1990