Estimation of legume symbiotic dependence: an evaluation of techniques based on 15N dilution uri icon

abstract

  • An N-15 isotope dilution technique, first proposed more than 40 years ago, is now widely used to estimate of the proportion of legume N derived from biological N-2 fixation. The proposed attributes of the method are critically examined. The problems associated with the non-uniform distribution of applied isotope and the traditional use of a non-fixing reference plant are elaborated. The use of models which do not require a reference plant is a relatively recent development in the evolution of the methodology. The characteristics and efficacy of the modelling approach are reviewed. (C) 1999 Elsevier Science Ltd All rights reserved.

publication date

  • 1999
  • 1999
  • 1999