Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos. uri icon

abstract

  • To enhance the level of resistance to insects in tropical maize germplasm we have developed techniques to successfully transform elite tropical maize inbred based on the activity of specific cryI proteins against four major maize pests - corn earworm, fall armyworm, southwestern corn borer and sugarcane borer. Constructs containing cryIAb or cryIAc synthetic genes were used. To generate transgenic plants we have established methods for biolistic bombardment and the selection and regeneration of immature embryos and calli from the elite tropical lines CML72, CML216, CML323, CML327 and hybrids. Transgenic plants resistant to the herbicide Basta(TM) contained the bands for the cry, bar and gus genes as detected by Southern blot analyses. A simple leaf bioassay presented varying levels of resistance to Southwestern corn borer of transgenic tropical maize carrying the cryIAc gene. Analyses of the progenies confirmed the sexual transmission of the introduced genes and their stable expression.
  • To enhance the level of resistance to insects intropical maize germplasm we have developed techniquesto successfully transform elite tropical maize inbredbased on the activity of specific cryI proteins againstfour major maize pests ? corn earworm, fall armyworm,southwestern corn borer and sugarcane borer. Constructscontaining cryIAb or cryIAc synthetic genes were used.To generate transgenic plants we have established methodsfor biolistic bombardment and the selection and regenerationof immature embryos and calli from the elitetropical lines CML72, CML216, CML323, CML327 andhybrids. Transgenic plants resistant to the herbicide BastaTMcontained the bands for the cry, bar and gus genesas detected by Southern blot analyses. A simple leaf bioassaypresented varying levels of resistance to Southwesterncorn borer of transgenic tropical maize carryingthe cryIAc gene. Analyses of the progenies confirmedthe sexual transmission of the introduced genes and theirstable expression

publication date

  • 1999
  • 1999
  • 1999
  • 1999