Hetero-diploid pollen grains that represent self-compatibility are incompatible with non-self receptors in tetraploid Chinese cherry (Prunus pseudocerasus Lindl) uri icon

abstract

  • Chinese cherry (Prunus pseudocerasus) has many natural tetraploid species within Prunus. The pollen grains of tetraploid Chinese cherry are hetero-diploid, the two S-haplotypes in the pollen are a combination of two of the four possible S-haplotypes. The abnormal segregation ratios of pollen-S indicate that a few hetero-diploid pollen grains could inactivate self-stylar S-RNase inside the pollen tube and grow better into the self-ovaries than to the others. In this study, three Chinese cherry cultivars, "Daiba" (S (1) S (2) S (5) S (8) ), "Taishanganying" (S (1) S (2) S (4) S (6) ), and "Laiyangduanzhi" (S (1) S (2) S (8) S (x) ), were used to investigate the inheritance of hetero-diploid pollen-S alleles in non-self receptors. Genetic analysis showed that the distribution of S-haplotypes is unequally expressed in self- and cross-pollinated progenies. The S (2) -haplotype, which is found with lowest frequency in all progeny plants, is a likely lethal mutation. Moreover, the number of individuals with two different S-haplotypes was also unequal in the two cross-pollinated progenies. Notably, the number of individuals with S (1) S (5) and S (1) S (8) genotypes was larger than other genotypes in the cross-pollinated progeny of "Laiyangduanzhi"aEuro parts per thousand x "Daiba", and the number of individuals with S (1) S (4) , S (1) S (6) , and S (4) S (6) genotypes was larger than S (2) -haplotypes in the cross-pollinated progeny of "Laiyangduanzhi"aEuro parts per thousand x "Taishanganying". These results indicate that only a few genotypes of hetero-diploid pollen grains have the capability to grow into the ovaries of "Laiyangduanzhi". Interestingly, the pollen grains with S (5) S (8) genotypes, which is self-compatible, was incompatible with the styles of "Laiyangduanzhi", while the pollen grains with S (1) S (8) , S (1) S (6) and S (4) S (6) genotypes, which are self-incompatible, were compatible with the styles of "Laiyangduanzhi".

publication date

  • 2014
  • 2014
  • 2014