Molecular characterization of Cryptosporidium spp. from wild rats and mice from rural communities in the Philippines uri icon

abstract

  • In order to examine the prevalence of Oyptosporidium in wild rodents in the Philippines and understand the role wild rodents play in the transmission of this parasite to humans and livestock, 194 fecal samples from wild rats and mice from Luzon and Mindoro islands were examined. Molecular screening at the 18S and actin gene loci identified an overall prevalence of 25.8% (95%CI: 19.8, 32.5). Sequence and phylogenetic analysis of both loci identified C parvum, C muris, C scrofarum, rat genotypes I-IV and a C suis-like genotype in the rat-derived isolates and is the first report of C suis-like and C. scrofarum in rats. Mixed infections were identified in 24% of the Oyptosporidium positive isolates. Rat genotypes II, III and IV showed high intragenotypic variation at the 18S gene locus compared to the actin locus. (C) 2013 Elsevier B.V. All rights reserved.

publication date

  • 2013
  • 2013
  • 2013