Genetics, fertility behaviour and molecular marker analysis of a new TGMS line, TS6, in rice uri icon

abstract

  • The thermosensitive genic male sterility (TGMS) system has greatpotential for revolutionizing hybrid rice production through simple,less expensive and more efficient seed production technology. For thesuccessful utilization of this novel male sterility system, knowledge ofthe breeding and fertility behaviour of a TGMS line is essential. In thisstudy, the fertility transformation behaviour, the critical fertility andsterility temperatures and the mode of inheritance of male sterility werestudied for a new TGMS line, TS6, identified at Tamil NaduAgricultural University, Coimbatore, India. The pollen and spikeletfertilities recorded on plants raised at fortnightly intervals revealedthat this line was completely sterile for 78 consecutive days (35/22 to32/23?C, maximum/minimum temperatures) and reverted to fertilewhen the temperature was 30/18?C. It remained fertile continuouslyfor 69 days and the maximum pollen and spikelet fertilities recordedwere 75 and 70%, respectively. The fertility was highly influenced bydaily maximum temperature followed by average and minimumtemperatures. It was not influenced by relative humidity, sunshinehours or photoperiod. The critical temperature inducing sterility andfertility was 26.7 and 25.5?C, respectively. The male sterility in TS6 wasinherited as a monogenic recessive in the F2 and BC1 populations ofTS6 · MRST9 as well as TS6 · IR68281B. Using bulked segregantanalysis on an F2 population of TS6 · MRST9, an RAPD marker,OPC052962, was identified to be associated with TGMS in TS6

publication date

  • 2004
  • 2004