Development of Pearl Millet Minicore Collection for Enhanced Utilization of Germplasm uri icon

abstract

  • Pearl millet (Pennisetum glaucum (L.) R. Br.)exhibits enormous genetic diversity in the globalgermplasm collection. The pearl millet core collection(~10% of the entire collection), with 2094accessions, is still large for precise evaluation toidentify parents for a genetic improvement program.A minicore collection of pearl millet comprising238 accessions was constituted by usingdata on 10 qualitative and 8 quantitative traits of2094 core collection accessions. The hierarchicalcluster analysis of data using a phenotypicdistance matrix resulted in 136 clusters. A proportionalsampling strategy with 10% or a minimumof one accession from each cluster wasused to form the minicore collection. The comparisonof data in the minicore and core collectionsusing various statistical parameters, suchas homogeneity of distribution for geographicalorigin and frequency classes of qualitative traits,means, median, within and between-accessionvariances, diversity index, and phenotypic correlations,indicated that almost the entire geneticvariation present in the core collection was capturedin the minicore collection. The possibleimpact of high between- and within-accessionvariability in pearl millet on maintenance ofgenebank accessions is discussed. With itsgreatly reduced size, the pearl millet minicorecollection can be used for precise evaluation oftraits of agronomic importance and biotic andabiotic stresses as well as mapping with molecularmarkers for identifi cation of trait-specifi cgermplasm and discovery of new genes

publication date

  • 2011
  • 2011