Efficient plant regeneration from embryogenic suspension cultures of sweetpotato uri icon

abstract

  • Using 15, Chinese and Japanese cultivars of sweetpotato, lpomoea batatas (L.) Lam., we succeeded in developing an efficient plant regeneration system from embryogenic suspension cultures. The embryogenic callus derived from shoot apices of the 15 cultivars, was used to initiate embryogenic suspension cultures in Murashige and Skoog (MS) medium containing 9.05 muM 2,4-dichlorophenoxyacetic acid (2,4-D). Rapidly proliferating and well-dispersed embryogenic suspension cultures were established. Cell aggregates 0.7-1.1 min in size from embryogenic suspension cultures were transferred to. solid MS medium supplemented with 9-05 muM of 2,4-D and formed embryogenic, callus with somatic embryos. The embryogenic callus with somatic embryos was further transferred to MS medium supplemented with 3.78 muM of abscisic acid, resulting in the germination of somatic embryos. Within 20 wk after the initiation, the frequencies of cell aggregates forming plantlets reached approximately 100% for the 15 tested cultivars. These plantlets, when transferred to, soil, showed 100% survival. No morphological variations were observed.

publication date

  • 2001
  • 2001