SSR and e-PCR Provide a Bridge Between Genetic Map and Genome Sequence of Potato for Marker Development in Target QTL Region uri icon

abstract

  • The aim of this study was to increase marker density in a previously identified late blight resistance QTL in a diploid potato population. SSR markers from the reference potato genome sequence were located by e-PCR in the QTL region in the chromosome 9 genetic map of the diploid potato population B3C1HP, and utilized to identify more candidate genes locating between the SSR markers. After verified by PCR and genetic mapping, two SSR markers and two DM candidate genes were mapped in the QTL peak in the maternal genetic map of B3C1HP. The new markers narrowed down the average 2-LOD support interval from 2.6 to 1.4 cM. The new markers on the LOD peak are valuable for fine mapping and positional cloning of the alleles in late blight resistance QTL. This study proves that combination of SSR and e-PCR is an effective way to develop markers in target QTL region.

publication date

  • 2015
  • 2015
  • 2015