AN ALTERNATIVE PRETREATMENT METHOD FOR MITOTIC CHROMOSOME OBSERVATION IN POTATOES uri icon

abstract

  • An alternative pretreatment method for mitotic chromosomes in potato root tips is described. Root tips were treated with a synthetic pyrethroid, a permethrine, {3-Phenoxybenzil (+/-) -Cis, trans, 3-(2,2 dichlorovinyl)-2,2-dimethyl ciclopropane carboxylate}. with concentrations of 10ppm, 100ppm, 200ppm, and 500ppm. Cold water and 0.02M 8-hydroxyquinoline were used for comparison at 4 C. Mitotic index was obtained for all treatments by using a standard squash method, and pretreatment with 100ppm of the pyrethroid demonstrated a large number of mitotic cells and well condensed chromosomes for a comprehensive observation in mitotic cells.

publication date

  • 1993
  • 1993