A method for isolation and purification of peanut genomic DNA suitable for analytical applications. uri icon

abstract

  • Numerous methods are available for isolation of plant genomic DNA, but inpractice these procedures are empirical due to variability in plant tissue composition. Consistentisolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematicdue to the presence of phenolic compounds and polysaccharides. Inconsistenciesin extraction results can be attributed to the age and growth stage of the plant material analyzed.Mature leaves have higher quantities of polyphenols, tannins, and polysaccharidesthat can contaminate DNA during isolation. We show that four published protocols couldnot be used to isolate peanut DNA of sufficient quality for PCR amplification or Southernhybridization. We have devised a new protocol that uses DEAE-cellulose purification toisolate peanut DNA useful for downstream applications

publication date

  • 2000
  • 2000