An RGA-Derived SCAR Marker Linked to PLRV Resistance from Solanum tuberosum ssp. andigena uri icon

abstract

  • A resistance gene analog (RGA)-derived sequence-characterized amplified region (SCAR) marker was successfully developed based on sequence homology with disease resistance genes of an AFLP molecular marker tightly linked to the Rl (adg) gene of Solanum tuberosum ssp. andigena. The new marker was designated as 'RGASC850' (RGA-derived SCAR) based on the size of the amplified fragment. 'RGASC850' could be efficiently used for monitoring introgression of Rl (adg) against backgrounds of improved gene pools with low likelihood of identifying false positives due to recombination. This SCAR proved to be highly predictive of Rl (adg) -based resistance, as it did not amplify potato leafroll virus (PLRV) resistance sources other than andigena, and thus would be useful in developing cultivars with complementary sources of resistance to PLRV. In addition, a cleaved amplified polymorphic sequence (CAPS) marker based on 'RGASC850' was developed capable of distinguishing genotypes carrying Rl (adg) . This CAPS marker would be useful for screening breeding populations derived from wide crosses, and confirming presence of the Rl (adg) gene in those parents amplifying the 'RGASC850' marker.

publication date

  • 2014
  • 2014