DNA fingerprinting detects genetic variability in the pearl millet downy mildew pathogen (Sclerospora graminicola). uri icon

abstract

  • Genetic variability in 6 host genotype-specific pathotypes of the pearl millet downy mildew pathogen, S. graminicola, was studied at the molecular level using mini- and micro-satellites. The results indicated that microsatellites (GAA)61, (GACA)4 and especially (GATA)4 were quite informative and showed high levels of polymorphism among the pathotypes. The 6 pathotypes could be classified into 5 groups based on the cluster analysis of their genetic similarities, thereby confirming the existence of distinct host genotype-specific virulence in S. graminicola pathotypes. The use of DNA fingerprinting to detect genetic variation in the downy mildew fungus of pearl millet was demonstrated for the first time

publication date

  • 1995
  • 1995