Ensuring Genetic Purity of Pigeonpea Hybrids by Incorporating a Naked-Eye Polymorphic Marker in A and B Lines uri icon

abstract

  • To enhance the productivity of pigeonpea (Cajanuscajan (L.) Millspaugh) a hybrid breedingtechnology, based on the cytoplasmic nuclearmale-sterility (CMS) system and partial naturaloutcrossing, is currently been used. However,there are diffi culties to maintain genetic purityof the hybrids and their parents. The incorporationof an easily identifi able morphologicalmarker (naked eye polymorphism (NEP)) couldbe used to determine seed purity. The morphologicalmarker selected for this study, obcordateleaf, is not present in cultivated pigeonpea; it isinherited as a single recessive gene and can beobserved soon after planting (approx. 6 wk). Toincorporate the obcordate leaf shape into hybridparents, the trait was transferred from the germplasmaccession ICP 5529 into male-sterile (Alines) and the corresponding maintainers (Blines). The hybrids derived from crosses involvingobcordate leaf A lines and normal leaf fertilityrestorers (R lines) were fully fertile and hadnormal lanceolate leaves; thus the differencebetween A line and hybrids was clear. The useof obcordate leaf as a NEP marker in pigeonpeawould contribute to preserve parental line purityand confi rm hybrid status

publication date

  • 2011
  • 2011