RFLP analysis of cytoplasmic male-sterile lines of pigeonpea [Cajanus cajan (L.) Millsp.] developed by interspecific crosses uri icon

abstract

  • Total DNA from three putative cytoplasmically male sterile (CMS) progenies derived from crosses between the wild species Cajanus sericeus and the cultivated species Cajanus cajan, and 7 C. cajan accessions (including 2 genetically male sterile lines) plus one accession of C. sericeus were compared for their RFLP patterns using maize mitochondrial DNA (mtDNA) specific probes. Three putative CMS progenies from the multiple cross genome transfer of pigeonpea lines (CMS 7-1, CMS 12-3, and CMS 33-1) showed hybridization patterns identical to that of C. sericeus when DNA was digested with EcoRI and HindIII and probed with maize mtDNA clones. The results suggested that these putative CMS progenies have the mitochondria of the female wild species parent. The hybridization patterns of the three male parental lines used in the development of the CMS progenies were similar in all the restriction enzyme-probe combinations except HindIII-atp6. The genetic male sterile lines, MS Prabhat and QMS 1 differed from each other in their hybridization pattern. The genomic DNA hybridization pattern of HindIII digested DNA from ICPL87 differed from the other pigeonpea lines when probed with the maize mtDNA clones. The cluster analysis of the hybridization data suggested the occurrence of variation in the mitochondrial genome even among the cultivated species

publication date

  • 1997
  • 1997