Future Prospects of Molecular Markers in Plants uri icon

abstract

  • The last ?30 years have witnessed a continuous evolution of newmolecular marker systems from restriction fragment length polymorphisms,random amplified polymorphic DNAs, and amplified fragmentlength polymorphisms to present-day popular marker systems suchas simple sequence repeats (SSRs), single nucleotide polymorphisms(SNPs), and diversity array technologies. Advent of low-cost and highthroughputsequencing technologies, commonly called next-generationsequencing (NGS) technologies have increased the speed of SSR andSNP discovery. NGS technologies in combination with restriction enzymesare now ready for detecting genome-wide polymorphism andnew marker systems like RAD-tag sequencing, genotyping by sequencingare becoming popular. It seems that NGS-based marker systems willbe dominating marker systems in future. These new emerging markersystems are expected to facilitate enhanced adoption of modern geneticsand breeding approaches like genome-wide association studies andgenome-wide selection that generally require markers at high-density incrop plants

publication date

  • 2012
  • 2012