Dataset for: Heritability in broad-sense for glycoalkaloid content in LBHT potato clones under warm conditions uri icon

abstract

  • In anticipation of the effects of global warming on potato cultivation in both tropical and subtropical environments. Since 2004, efforts have turned to the development of a new group in Population B with improved adaptation to warm environments, resistance to late blight and virus, mid-season maturity (90 day growing period under short day length conditions), adaptation to mid elevations, low glycoalkaloids content, along with economically important traits such as high tuber yield, quality for table and industry, denominated LBHT (late blight, heat tolerance). In this context, the broad-sense heritability for accumulation of glycoalkaloids in tubers of LBHT clones with resistance to late blight and heat tolerance under high temperature conditions, was estimated. In 2014-2015, three experiments took place at San Ramon, La Molina and Majes in Peru. In this experiments, 20 LBHT clones with varieties Unica and Desiree as controls were evaluated. The RCB design was used, with three replications of 20 plants each. In all experiments, the number of emerged plants and the number and weight of marketable and non-marketable tubers per plot were recorded. Also, tuber samples from each clone in each replication in all three experiments were taken. The following variables were calculated: tuber yield, number of tubers per plant and the average tuber weight. Besides, the total glycoalkaloid concentration was estimated using the extraction and purification method of Wang et al. 1972, and the spectrophotometry determination indicated by Hellenas et al. 1986. Freeze dried and milled samples coming from peeled tubers were extracted using methanol and chloroform and then transferred to 5% acetic acid solution. And next the extract was purified using ammonium hydroxide and centrifugation. Lastly, the pellet reacted with orthophosforic acid and total glycoalkaloid concentration was determined by spectrophotometry using the standard curve of a-chaconine as reference. Furthermore, Pearson's correlation was used to measure the linear association between tuber yield and glycoalkaloid concentrations and for statistical and genetic analyses SAS for Windows, version 9.4 (SAS Institute Inc.) software was used

publication date

  • 2018

geographic focus