Genetic Linkage Map Construction in Pearl Millet Using SSR Markers uri icon

abstract

  • For pearl millet production in the semi-arid tropics, Sclerospora graminicola is a major bioticconstraint causes Downy mildew. The pathogen is heterothallic and frequent recombinationleads to evolution of new virulent populations. Identification of resistance to new virulent isolatesis a prerequisite for resistance breeding. A very good understanding of ability to manipulateoligogenic and quantitative traits is offered to plant breeders by recent advances in geneticmarker technology. In the present investigation genotypic data generated for a total of 88 markerloci (39 Genomic SSRs and 49 EST SSRs) were used to construct a linkage map of the pearlmillet mapping population of 188 F8 RIL progenies based on the cross ICMB 89111-P6 x ICMB90111-P6. A skeleton linkage map of seven linkage groups with a total map length of 725.5 cM(Haldane units) was constructed using data from 74 marker loci for 188 RILs using JoinMap atLOD threshold value of 5.0 and MapMaker/Exp version 3.0b and map was drawn using MapChart 2.2. The map length of individual linkage groups ranged from a minimum of 32.1cM (LG3)to a maximum of 140.2 cM (LG1). The average inter marker distance was 9.8 cM, with anaverage density of 0.102 markers/cM. The total number of mapped loci per linkage group (LG)ranged from 5 on LG3 to 23 on LG1

publication date

  • 2017