Haplotype for drought responsive genes in pigeonpea uri icon

abstract

  • Pigeonpea is a resilient crop which is relatively drought tolerantcompared to many other legumes. To understand themolecular mechanisms of this unique feature in pigeonpea, 51genes were selected using the Hidden Markov Models (HMM).These genes were validated for their expression on parents ofmapping populations (ICPL 151, ICPL 8755, and ICPL 227) segregatingfor drought tolerance. A total of 10 differentially expressedgenes that showed ?2-fold up-regulation in the higherdrought tolerant genotypes was identified. Further analysis ofthe 10 genes revealed that they encode four different classes ofproteins. These include plant U-box protein (four genes), universalstress protein A-like protein (four genes), cation/H(+)antiporter protein (one gene) and an uncharacterized protein(one gene). Subsequently, these 10 candidate genes were usedfor haplotype analysis in whole genome re-sequencing data of292 lines. As a result, 10 to 56 haplotypes were identified forthe selected 10 genes. In order to validate the haplotype results,a defined set of lines is being phenotyped for inducedwater stress using polyethylene glycol (PEG) at seedling stagealong with the non-stressed control seedlings. Phenotypingdata together with the haplotype information of the targetedcandidate genes will be useful for the identification of superiorhaplotypes of genes. The lines possessing superior haplotypesof the candidate genes could be used in genomics-assistedbreeding programs for development of more drought tolerantlines

publication date

  • 2017