Towards defining heterotic pools for accelerating hybrid breeding in pigeonpea uri icon

abstract

  • Recently released pigeonpea hybrids for cultivation in farmers?fields have shown their potential to elevate the yield levels. Forinstance, the world?s first grain legume hybrid of pigeonpeaICPH 2671 showed 47% yield advantage over the check varieties.Generally, the development of such hybrids is based onselection efficiency of breeding program; breeders make thousandsof random crosses between cytoplasmic male sterile(CMS) lines and tester lines. In order to enhance the selectionefficiency, genomic diversity along with the phenotyping datahave been used for predicting the best possible parental combinations.This approach has been successfully used in definingheterotic pools in many crop species such as maize, rice, sunflower,and rapeseeds.. With an aim to define heterotic pools inpigeonpea, a set of 104 parental lines (09 CMS, 13 maintainersand 82 restorers) have been re-sequenced following whole genomere-sequencing (WGRS) approach. WGRS yielded a totalof 511 GB data with the coverage ranging from 5X to 10X. Atotal of 3.4 million SNPs have been identified across parentallines. Structural variations such as copy number and presence/absence variations have been also identified. In parallel, theseparental lines have been used to develop test crosses in factorialmating design. F1 hybrids along with parental lines werephenotyped for yield and yield related traits at two locationsin India, namely ICRISAT, Telangana State and ARS-Gulbarga,Karnataka. The availability of genome-wide SNP variationscombined with the phenotypic data will be used for deployinggenomic selection to define hetereotic pools in pigeonpea foraccelerating hybrid breeding program

publication date

  • 2017