Next generation sequencing approaches for understanding genetic mechanism of drought tolerance in Valencia Peanut uri icon

abstract

  • Valencia peanuts are highly susceptible to drought stress.The yield reduction due to stress can result in more than 50%reduction resulting in high aflatoxin contamination. In ourpresent study we have compared Valencia-C genotype witha highly drought tolerant runner type peanut C76-16 usingtranscriptome sequencing to investigate tissue-specific geneexpression and response to abiotic stress. We evaluated thetwo genotypes under field conditions under irrigated and stressplots in 4 replications. We found that genotype C76-16 outperformedyield compared to Valencia-C under drought stressconditions with only a 30 % reduction in yield while Valencia-Cshowed a higher loss of up to 70%. Each year the yield variedwidely due to the environmental factors but C76-16 was consistentlyranked among the top and suffered a minimal loss ofyield suggesting that it is best suited for the erratic climaticconditions of southwest U.S. Leave samples were harvestedfrom the control and stress conditions and used for RNA isolationand sequencing. A total of 340 million paired-end readswere generated and were used to find differentially expressedgenes and transcripts between tolerant and susceptible genotypes.The pathways and candidate genes involved in droughttolerance will provide basic information and expression markersfor drought tolerance in peanut. We will present our findingson genic content and tissue-specific gene expression anddiscuss the challenges and opportunities of unifying transcriptsequence data for the peanut community

publication date

  • 2017