Evaluating human exposure to aflatoxins: A case study on aflatoxinalbumin adduct levels in end stage liver disease patients in India uri icon

abstract

  • Aflatoxin B1, a secondary metabolite of Aspergillus flavus and A. parasiticus,is a frequent contaminant of several food crops. The main objective of thisstudy was to know the effect of post exposure of aflatoxin in humans throughconsumption of contaminated foods. The aflatoxin-albumin level of selectedhuman populations in India were analysed. A total of 673 blood samples werefurther analyzed by Indirect competitive Enzyme linked immunosorbent assay(IC-ELISA) method. The samples were collected along with clinical profile,demographic and food consumption data from the individuals with differentstages of liver disease. The severity of the liver disease was calculated basedon the ?Model for End stage Liver Disesae? (MELD) score. The resultsindicated 86 of 673 samples have concentration of aflatoxin-albumin adductranged between 2.5 to 677 pg mg-1 of albumin with mean adduct level 181.9±5 and SD 1.49 ± 7. At 5% level of significance pb value 0.039 (<0.05)indicates presence of aflatoxin B1-lysine adducts and hepatitis B hassynergistic effect on liver damage especially in end stage liver disease basedon model for end stage liver disease (MELD). This shows that there isconsiderable intake of aflatoxin through contaminated food. Interestingly theconcentration of aflatoxin-albumin was high and this along with the HBVpositivity was found to be contributing to the severity of liver disease and leadto decompensated liver disease

publication date

  • 2013