Analysis of Genetic Diversity in Pongamia (Pongamia pinnata (L)Pierrre) using AFLP Markers uri icon


  • In recent years, Pongamia has been considered as important renewable source of biodiesel, however not much molecularinformation is available in this species. Molecular characterization of this legume tree will enhance our understanding inimproving the optimal yields of oil through breeding and enable us to meet the future demands for biodiesel. To assess themolecular genetic diversity in 48 Pongamia pinnata accessions collected from six different states of India, amplified fragmentlength polymorphism (AFLP) marker system was employed. Five AFLP primer combinations produced 520 discerniblefragments, of which 502 (96.5%) were polymorphic. AFLP primer informativeness was estimated evaluating four parametersnamely polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP).In total, 51 unique fragments were detected of which 19 unique fragments were observed with primer combination E-ACG /M-CTA. Although neighbour joining (NJ) method did not group accessions strictly according to their region of collection, agood level of genetic diversity was observed in examined germplasm. However, accessions collected from Karnataka showedcomparatively higher diversity than accessions from other states. The diverse accessions identified in this study may beuseful in Pongamia pinnata improvement to meet the future demands of biodiesel

publication date

  • 2010