Bio-active Secondary Metabolities from PGPR and Botanicals uri icon

abstract

  • ICRISAThas a large collection of-bacteria, fungi and actinomycetes with agriculturally beneficial traits isolated from various sources of composts and rhizosphere soil samples from sorghum, rice and pigeonpea crops, in addition to potent botanicals. At least 1500 accessions of plant growrth promoting microorganism (PGPM viz. 89 phosphate solublizers, 252 siderophore producers, 198 cellulose degraders, 490 nitrogen fixers, 350 antagonists, 101 entomopathogens and 20 fluorescent Pseudomonads) have been isolated .from the above sources ill addition to 17 botanicals capable of managing Helicoverpa armigera and Spodoptera .litura, the two' most devastating insect pests of many crops, Bio-active secondary metabolites of the potent PGPM and botanicals (particularly on Anona, Datura, Pongamia, Parthiniuim, Gliricidia, Neem and Jatropha) responĀ¬sible for managing H. armigera and S. litura) and antagonistic to five disease causing fungi (Fusarium oxyspor:um f. Sp. ciceri, F, udum, F. solani, Sclerotium rolfsi and .Macrophomina phaseolina.) were studied . Secondary metabolites of the potent PGPM strains and botanicals were purified by solvent partitioning, solid phase extraction, TLC and reversed-phase open column chromatography. Stages in purification were monitored by a live/dead assay employing neonates of H. armigera and S. litura or plant pathogenic fungi. Final purification will be done in HPLC and the purified active compound(s) will be identified by mass spectrometry and nuclear magnetic resonance studies. Purification of the secondary metabolites from the above PGPM and botanicals are on and results will be discussed in presentation

publication date

  • 2009