Chickpea uri icon

abstract

  • Pulses form a major source of protein, particularly for vegetarian diet, and chickpea ranks among the leading legumes in this regard. Albeit considerable efforts at national and international levels, productivity of the chickpea crop has been stagnated for long due to the major biotic and abiotic constraints caused by Ascochyta blight, Fusarium wilt, pod borer, drought, and cold. Though many wild annual Cicer species possess a wealth of agronomically desirable donor genes, particularly those conferring resistance to stresses, they are sexually incompatible with the cultivated species. Transgenic approach is, therefore, a potential alternative means for incorporation of desirable alien genes into chickpea. This chapter highlights various dimensions of the international efforts in producing transgenic chickpea and provides a comprehensive perspective of strategies and achievements. Development of transgenic chickpea includes different aspects such as locating genes for particular traits from available chickpea germplasm, their isolation, making suitable constructs to transfer into chickpea through modifying them by adding marker gene, promoter sequence, and termination sequence for their effective expression, and finally transformation followed by an efficient regeneration protocol. This technology will have a very important role in developing newer varieties of chickpea with desired traits such as insect resistance, disease resistance, and drought/cold resistance

publication date

  • 2008