Differential Responses of Proline, Ion Accumulation and Antioxidative Enzyme Activities in Pearl millet (Pennisetum glaucum (L.) R. Br.) lines Differing in Salt Sensitivity uri icon

abstract

  • Ten-day-old seedlings of pearl millet (Pennisetum glaucum (L.) R. Br.) mapping populations differing in their salinity tolerance levels were exposed to 0 and 150 mM NaCl concentrations for short durations of time (0 to 144 h) to assess the pattern of accumulation of proline, glutathione, Na+, K+, Ca2+ and Cl- contents and their antioxidative enzyme activities. Salt-tolerant lines accumulated more proline and K+ than the susceptible ones pointing their accumulation as a possible mechanism of salt tolerance. Specific activities of CAT, SOD and GR were higher in tolerant compared to the sensitive lines under salt stress conditions. High GST activity was noticed in the moderately tolerant line while the increase was transient (till 48 h) in the tolerant line. Lipid peroxidation as measured by MDA levels remained more or less same in the salt-sensitive line ICMB 90111, while it increased considerably in the tolerant line under salt stress till 96 h. The above comparative studies suggest that salt-tolerant and salt-sensitive lines of pearl millet possess differential oxidative components of both enzymatic and non-enzymatic machinery for scavenging ROS generated during salt stress

publication date

  • 2007