Rapid and reliable screening technique for root traits in chickpea uri icon

abstract

  • A set of 257 chickpea recombinant inbred lines (RILs) were derived from a cross of a prolific root system (ICC 4958) and an agronomically preferred variety (Annigeri). The RILs were developed for identifying QTLs associating with desirable root traits and were grown in tall PVC cylinders (120 cm height) filled with the vertisol and sand mixture under two initial soil water contents, 100% field capacity (irrigated) and 70% field capacity (stressed) during 2001 post-rainy season. The same chickpea RILs were also grown in an experimental field under rainfed condition. At 35 days after sowing (DAS) in cylinder cultures, there were significant variability in shoot and root dry weights and root length density among the RILs. ICC 4958 showed larger shoot and root growth than Annigeri in field and water stressed cylinder systems but not in irrigated cylinder. A significant positive correlation was observed in the root growth between the field and cylinder conditions and significant G × E interaction in the root dry weight and length density was also detected in irrigated cylinder. This cylinder system with 70% field capacity can be used as powerful and rapid phenotyping tool for root traits in large scale screening of germplasm or mapping populations

publication date

  • 2007