Survival and development of Helicoverpa armigera on artificial diet impregnated with lyophilized leaf and pod powder of different chickpea genotypes uri icon

abstract

  • Host plant resistance is one of the components for minimizing the damage by the noctuid pod borer, Helicoverpa armigera in chickpea. However, due to variations in H. armigera infestations in space and time, it becomes difficult to evaluate the test material in under natural infestation. Therefore, we evaluated the diet impregnation assay to evaluate chickpea genotypes for resistance to H. armigera. Ten-day old larvae weighed highest on the standard diet, followed by those reared on diets with leaf powder of the susceptible checks, ICC 4918 and ICCC 37. Larval weights were significantly lower in larvae reared on the leaves/pods of ICC 12476, ICC 12477, ICC 12478, ICC 12479 and ICCV 2 as compared to those reared on the susceptible check, ICC 12426. The larvae reared on artificial diet impregnated with lyophilized leaf and/or pod powder of ICC 12475, ICC 12476, ICCV 2, and ICC 12479 also weighed significantly lower than those fed on diets with ICC 12426, and ICC 3137. Of these, larval period was prolonged on fresh leaves/pos of ICC 506, and in diets with pod powder of ICC 3137, ICC 12479, ICCV 2, and ICC 506. Comparatively lower pupal weights were recorded in larvae reared on fresh leaves/pods and on artificial diets with leaf and pod powder of ICC 12476, ICC 12477, ICC 12478, and ICC 506 as compared to those on ICCC7 37; while larval survival, pupation, and adult emergence were lower on the fresh leaves/pod and on artificial diets with leaf and pod powder of ICC 12476, ICC 12477, ICC 12478, and ICC 506 as compared to the insects reared on the susceptible checks, ICC 37 and ICC 4918. Larval survival and development were also adversely affected on the F1 hybrids based on these genotypes as compared the susceptible check, ICCC 37. There was a significant reduction in fecundity of insects reared on the fresh leaves/pods and on artificial diets with leaf and pod powder of ICC 12476, ICC 12477, ICC 12478, ICC 12479, and ICC 506 as compared to the insects reared on ICCV 2, ICC 4918, and ICCC 37. The results indicated that the antibiosis to H. armigera in chickpea is expressed in terms of slower development, and reduced survival and fecundity. Survival and development of H. armigera of fresh leaves/pods and on diets with lyophilized leaf and pod powder of different chickpea genotypes were highly correlated suggesting that diet impregnation assay can be used to assess antibiosis component if resistance to H. armigera in chickpea

publication date

  • 2006