Biological activity of lectins from grain legumes and garlic against the legume pod borer, Helicoverpa armigera uri icon

abstract

  • The effects of lectins from field bean (Phaseolus vulgaris), pigeon pea (Cajanus cajan), chickpea (Cicer arietinum), garlic (Allium sativum) and snowdrop (Galanthus nivalis) lectin on the growth and development of H. armigera were studied. The lectins were bio-assayed against the neonate larvae of H. armigera by treating the surface of the artificial diet. The differences in larval weights among the diets with different lectins were not significant. The weight of the pupae reared on a diet containing garlic lectin II (from transgenic tobacco) was significantly lower (283.81 mg per larva) than that of the untreated control diet (325.00 mg per larva). The pupal period of the insects reared on diets containing lectins from field bean, pigeon pea, chickpea and garlic was significantly shorter than that reared on the untreated control diet. The differences in percentage pupation and adult emergence were not significant. However, less than 60% pupation was recorded in diets treated with lectins from pigeon pea, chickpea in 60% ammonium sulfate solution, garlic, and garlic lectin extracted from transgenic plants compared to 76.67% in the untreated artificial diet. Adult emergence ranged from 33.33% in diets treated with pigeon pea and garlic lectin to 46.67% in the untreated control diet. The sex ratio (males:females) was affected adversely in diets treated with lectins from field bean and pigeon pea

publication date

  • 2005