Transmission and properties of a new luteovirus associated with chickpea stunt disease in India uri icon

abstract

  • Several luteoviruses are involved in the chickpea stunt disease (CpSD) etiology. Earlier surveys identified a new luteovirus, Chickpea stunt disease-associated virus (CpSDaV), widely associated with CpSD in India. This study investigated its properties and host range, and the aphid vector species involved in its transmission. Purified CpSDaV preparations have typical luteovirus properties: isometric particles of 28 nm in diameter, a single coat-protein of 24.2 kDa and one RNA species of c. 5 kb. Polyclonal antiserum produced to purify CpSDaV preparations was useful for virus detection. CpSDaV which is serologically related to Beet western yellows virus (BWYV), has a host range distinct from that of BWYV, and is vectored by Aphis craccivora. Based on these properties and sequence homology, CpSDaV is a new species in the genus Polerovirus, family Luteoviridae. CpSDaV inoculated chickpea plants developed typical stunt disease symptoms, confirming its role in CpSD etiology

publication date

  • 2004