Techniques for diagnosis of Pseudomonas solanacearum and for resistance screening against groundnut bacterial wilt: Training Manual-1 uri icon

abstract

  • Bacterial wilt, caused by Pseudomonas solanacearum, is a widespread and destructive disease of such economically important crops as banana, egg-plant, groundnut, pepper, potato, tobacco, and tomato, in the tropics, sub-tropics, and warm temperateregions. The pathogen has a wide host range and an exceptional ability to survive in th roots of nonhost plants and in the soil. This manual presents techniques for detection, isolation, and identification of all variants of the wilt pathogen. The use of enzyme-linked immunosorbent assay (ELISA) todetect the bacterium in plant tissues, seed, and soil is emphasized. DNA-based diagnostics for P. solanacearum are described, as are an infectivity titration technique to determine the virulence of the wilt pathogen, and several inoculation techniques used in evaluating host-plant resistance togroundnut bacterial wilt

publication date

  • 1995