Tissue culture response of CIMMYT elite bread wheat cultlvars and evaluation of regenerated plants uri icon

abstract

  • Six elite CIMMYT bread wheat genotypes Pavon 76, Seri M82, Opata M85, Mochis T88, BaviacoraM93, and the advanced line Attila were evaluated for their response to in vitro tissue culture. Donor plantswere grown at EI Batan and CIANO experiment stations in Mexico in 1992. Immature embryos, about 1.0mm in length, were excised and placed scutellum side up on callus initiation media. Basal N6 mediumsupplemented with dicamba (E1), MS medium containing 2,40 (E3), or MS medium containing 2,40 plusdifferent amino acids (E5) was used for callus initiation and maintenance. Plant regeneration and rootingwere achieved on basal MS medium with IAA and BAP.Embryogenic calli with regeneration potentials of 10-92% were obtained on E3 and E5 media;each embryo produced 5-50 plants. A total of 800 regenerated plants were transferred to pots in thegreenhouse for evaluation and seed production. Of the regenerated plants, 85% were morphologicallynormal. reached full maturity, and produced seeds. Seeds (R1) of 360 plants regenerated from MochisT88, Baviacora M93, and Altila were planted in the field. Field observations indicated that embryoscultured on E3 medium resulted in 29% of the progeny producing deviating plants, compared with 21 % ofthose grown on E5 medium. These results will form the basis for future efforts aimed at transformingCIMMYT bread wheat varieties.IntroductionSix elite CIMMYT bread wheat genotypes Pavon 76, Seri M82, Opata M85, Mochis T88, BaviacoraM93, and the advanced line Attila were evaluated for their response to in vitro tissue culture. Donor plantswere grown at EI Batan and CIANO experiment stations in Mexico in 1992. Immature embryos, about 1.0mm in length, were excised and placed scutellum side up on callus initiation media. Basal N6 mediumsupplemented with dicamba (E1), MS medium containing 2,40 (E3), or MS medium containing 2,40 plusdifferent amino acids (E5) was used for callus initiation and maintenance. Plant regeneration and rootingwere achieved on basal MS medium with IAA and BAP.Embryogenic calli with regeneration potentials of 10-92% were obtained on E3 and E5 media;each embryo produced 5-50 plants. A total of 800 regenerated plants were transferred to pots in thegreenhouse for evaluation and seed production. Of the regenerated plants, 85% were morphologicallynormal. reached full maturity, and produced seeds. Seeds (R1) of 360 plants regenerated from MochisT88, Baviacora M93, and Altila were planted in the field. Field observations indicated that embryoscultured on E3 medium resulted in 29% of the progeny producing deviating plants, compared with 21 % ofthose grown on E5 medium. These results will form the basis for future efforts aimed at transformingCIMMYT bread wheat varieties.Introduction

publication date

  • 1995