A Technique for Phytophthora drechsleri f. sp. cajani Sporangia and Zoospores Production uri icon

abstract

  • Cultures on V-8 juice agar medium were incubated for 5 d at 30°C, then 5 mm diam. discs were removed from the periphery of the colonies and floated in clear V-8 juice and sterile distilled water (1:5) for a further 24 h at 30° under fluorescent light. The mycelial discs were rinsed twice in sterile distilled water and incubated again under the same conditions in sterile distilled water. Abundant sporangia were produced in 16 h and suspensions of c. 50?000 zoospores/ml were obtained

publication date

  • 1994